High efficiency PCR clean up with AHN myTube® spin columns

After running a polymerase chain reaction (PCR) it is often necessary to separate the PCR products from the reaction mix (nucleotides, primers, enzymes, and buffer), a process commonly referred to as PCR clean up. The AHN myTube® spin column system streamlines PCR clean up by providing an efficient DNA recovery process. In this article, we will highlight some of the excellent features of AHN myTube® spin columns that make them the ideal PCR clean up solution for your lab.

High-affinity DNA binding

The initial stages of PCR clean up using spin columns usually involve the use of commercially available kits to bind DNA to a filtration membrane. The PCR reaction mix is combined with a binding solution and centrifuged through a spin column to separate the DNA from the other components of the PCR mix. AHN myTube® spin columns with glass fiber filters provide a high-affinity DNA binding matrix for PCR clean up. These silica-based filters offer maximum nucleic acid retention during the binding step with an efficiency upwards of 99.99%.

Excellent DNA retention during washing

DNA binding is followed by one or more wash steps that ensure that the PCR clean up process separates the DNA from any residual components of the reaction mix. The success of the wash step(s) in PCR clean up depends on the DNA remaining bound to the filter membrane of the spin column while the other components of the reaction mix are washed into the collection tube. The glass fiber filters of AHN myTube® spin columns have high-efficiency nucleic acid retention throughout the wash steps of PCR clean up, minimizing the chances of DNA loss. The glass fiber filters achieve this by selectively binding to DNA in the presence of chaotropic salts. AHN myTube® Spin Columns with glass fiber filters help you take the guesswork out of PCR clean up.

Reversible DNA binding for easy elution

The last stage of PCR clean up using AHN myTube® spin column involves removal of bound DNA from the glass fiber filters – a step commonly referred to as elution. The bound DNA is easily eluted in a low-salt environment by simply adding nuclease-free water to the spin column and centrifuging it. This makes quick work of PCR clean up while maximizing DNA recovery. With AHN myTube® Spin Column PCR clean up is fast, non-toxic, and easy for any life scientist to accomplish.

Centrifuge-optimized Spin Columns

As centrifugation is a pivotal part of the PCR clean up process high-quality centrifugation tubes are necessary. Constructed from high-grade polypropylene, AHN myTube® spin columns and receiver tubes can be used in a variety of centrifuges with excellent results. Our custom formulation of high-grade polypropylene is tailored to ensure the spin columns and receiver tubes do not interact with the reaction components. This maximizes DNA binding at the filter as well as the collection of flow-through in the wash steps of PCR clean up.

Convenient volume control

The receiver tubes of the AHN myTube® spin column system have graduation marks that make it convenient to visualize the volume of fluid collected after each spin cycle. This feature makes it easy to audit the flow through to quickly catch pipetting errors as PCR clean up proceeds. The receiver tubes are supplied in 1.5-2.0mL capacities while retaining a universal fit with AHN myTube® spin columns.

Order your AHN myTube® spin columns today

If you would like to try out the AHN myTube® spin column system for your own PCR clean up, we would be happy to send you a free test sample. Click here to find out more information on how to place your order for the ultimate AHN myTube® spin column system on the market.

See AHN myTube® spin columns for PCR clean upSee AHN myPlate® microfilter plates for PCR clean up

Über die AHN Biotechnologie GmbH

AHN Biotechnologie GmbH is a company located in Nordhausen – Germany, which is specialized in the development, manufacturing and distribution of LIQUID HANDLING PRODUCTS for the use in laboratories worldwide. The product range includes pipette tips, filter tips, pipettes, tubes and products for filtration and purification of biomolecules. For over 20 years AHN Biotechnologie GmbH has been an OEM manufacturer of consumables for many top life science brands and distribution companies in Germany and worldwide. Our state of the art production facility meets and exceeds the strict requirements of the most well-known brands, we are supplying for. Our know-how within pipette tips’ production has now been strengthened by the implementation of newly developed Swiss molds, leading to the launch of new tips’ lines, matching the strict standards of modern lab industry. Our goal is to offer the market’s best value for money on pipette tips and other AHN products. [url=https://ahn-bio.de/?utm_source=PresseBox&utm_medium=PresseBox&utm_campaign=PresseBox]Click here for more information[/url]

Firmenkontakt und Herausgeber der Meldung:

AHN Biotechnologie GmbH
Uthleber Weg 14
99734 Nordhausen
Telefon: +49 (3631) 65242-0
Telefax: +49 (3631) 65242-90

Magdalena Babut-Carstensen
Marketing Manager
Telefon: +49(0) 3631 65242-0
E-Mail: mb@cappahn.com
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